Donald Trump banned the US government from giving funding to health groups that offer abortion counselling.
The President signed an order reinstating the “global gag rule”, which denies US foreign aid to groups who “perform or actively promote” terminations.
He was photographed signing the bill in the Oval Office, flanked by eight members of his staff, all of whom are men.
Vice President and anti-abortion campaigner Mike Pence, chief of staff Reince Priebus, his son-in-law Jared Kushner and top advisor and former far-right website owner Steve Bannon were all present.
There did not appear to be any women present for the signing.
On his first full working day as President, Trump also signed orders on withdrawing the US from the Trans-Pacific Partnership (TPP) trade deal and a hiring freeze for all departments but the military.